Total RNA Blood Kit (Lab-Aid) is designed for automated isolation and purification of total RNA from fresh whole blood and cultured cells with magnetic particle technology. The product is intended to be used by professional users, such as technicians and physicians who are trained in molecular biological techniques. The product in combination with Lab-Aid 824s/808 Nucleic Acid Extraction System provides high-quality total RNA for downstream applications, such as amplification or other enzymatic reactions. The Lab-Aid 824s Nucleic Acid Extraction system performs all steps of the sample preparation, which can extract up to 24 samples in a single run. The Lab-Aid 808 Nucleic Acid Extraction system performs all steps of the sample preparation, which can extract 8 samples in a single run.

Principle

The automated purification of nucleic acids is based on magnetic particle technology, which combines the speed and efficiency of the instrument with the convenient handling of magnetic particles. The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples. The purification procedure comprises 4 steps: lysis, binding, washing, and elution.
Lysis: The samples are lysed first with lysis buffer to release the nucleic acids.
Binding: Binding buffer is added to the lysed samples to adjust binding conditions. Lysates are thoroughly mixed with magnetic particles to allow optimal adsorption of nucleic acids to the silica surface. Salt and pH conditions ensure that protein and other contaminants, which can inhibit PCR and other downstream enzymatic reactions, are not bound to the magnetic particles.
Washing: While the nucleic acids remain bound to the magnetic particles, contaminants are efficiently washed away during a sequence of wash steps.
Elution: Highly pure nucleic acids are eluted in elution buffer.

Sample Type

Fresh whole blood, cultured cells

The kit is used with Lab-Aid 824s Nucleic Acid Extraction System and Lab-Aid 808 Nucleic Acid Extraction System.

Order Information

CE-IVD Marked