Pathogen Nucleic Acid Extraction Kit (Lab-Aid 896) is designed for automated isolation and purification of nucleic acids from bronchoalveolar lavage fluid (BALF), swabs, etc. with magnetic particle technology. The product is intended to be used by professional users, such as technicians and physicians who are trained in molecular biological techniques. The product in combination with Nucleic Acid Extraction System (Model: Lab-Aid 896) provides high-quality nucleic acids for downstream applications, such as amplification or other enzymatic reactions. The Nucleic Acid Extraction System (Model: Lab-Aid 896) performs all steps of the sample preparation, which can extract up to 96 samples in a single run.

Principle

The automated purification of nucleic acids is based on magnetic particle technology which combines the speed and efficiency of the instrument with the convenient handling of magnetic particles. The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples. The purification procedure comprises 4 steps: lysis, binding, washing, and elution.
Lysis: The samples are lysed first with lysis buffer to release the nucleic acids.
Binding: Binding buffer is added to the lysed samples to adjust binding conditions. Lysates are thoroughly mixed with magnetic particles to allow optimal adsorption of nucleic acids to the silica surface. Salt and pH conditions ensure that protein and other contaminants, which can inhibit PCR and other downstream enzymatic reactions, are not bound to the magnetic particles.
Washing: While the nucleic acids remain bound to the magnetic particles, contaminants are efficiently washed away during a sequence of wash steps.
Elution: Highly pure nucleic acids are eluted in elution buffer.

Sample Type

Swab, bronchoalveolar lavage fluid (BALF)

The kit is used with Lab-Aid 896 Nucleic Acid Extraction System.

Order Information

CE-IVD Marked