The Mycobacteria Identification Kit (Sanity 2.0) (Abbreviation: Sanity Myco) is a cartridge-based test kit for identifying 19 clinically relevant mycobacteria from sputum samples, including:

• M. tuberculosis complex
• M. chelonae
• M. scrofulaceum
• M. bovis
• M. smegmatis
• M. abscessus
• M. kansasii
• M. gordonae
• M. lentiflavum
• M. simiae
• M. nonchromogenicum
• M. terrae
• M. marinum/M. ulcerans
• M. fortuitum
• M. avium
• M. intracellulare
• M. xenopi
• M. malmoense
• M. szulgai

This kit is designed to run on Sanity 2.0, which integrates nucleic acid extraction, real-time polymerase chain reaction (rt-PCR), analysis of melting curve and the result interpretation into a fully automatic instrument (all-in-one machine).

Principle

The Sanity Myco employs dually labeled probes to target the excess single-stranded amplicons generated by asymmetric PCR; then uses melting curve analysis to generate the minus derivative of the fluorescence intensity versus temperature, and according to the specific melting temperature (Tm) to gain the information of the sequence. In the case of the presence of a specific target, a melting peak will arise at a corresponding position, and the Tm value will associate with a specific mycobacteria. Moreover, a fragment of the Arabidopsis thaliana sucrose-proton symporter 2 (SUC2) gene was included as an internal Positive Control (IPC) for indication of PCR inhibition.

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