The STI-8 Real-time PCR Reagent (Sanity 2.0) is used to identify 8 commonly seen sexually transmitted infection (STI) pathogens in vaginal or cervical swab, which include:

• Herpes Simplex Virus – Ⅰ (HSV-1)
• Herpes Simplex Virus – II (HSV-2)
• Trichomonas vaginalis (TV)
• Ureaplasma urealyticum (UU)
• Haemophilus ducreyi (HD)
• Neisseria gonorrhoeae (NG)
• Treponema pallidum (TP)
• Chlamydia trachomatis (CT)

This reagent uses probe-based fluorescence melting curve analysis, which basic principles are: add probe identified with fluorophore and quencher to PCR system, amplify oligonucleotide sequence that complements probe sequence during PCR process, add melting curve analysis process upon completion of amplification, and have real-time monitoring on changes in fluorescence value at the same time. Specific melting temperature (Tm value) generated from hybridization of PCR product and fluorescence probe can be obtained through calculation of negative derivative of fluorescence value and temperature, and thus achieve a single-tube for differentiation of 8 STI pathogens. Meanwhile, the detection kit uses human internal control (human RNase P) for quality control over extraction and amplification system.

The reagent should be operated on Sanity 2.0 System, the results are interpreted automatically.

For Research Use Only. Not for use in diagnostic procedures.