The Respiratory Pathogen Panel (RPP4D) is a multiplex real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay developed for the in vitro qualitative detection and differentiation of viral RNA—specifically influenza A, influenza B, respiratory syncytial virus (RSV), and SARS-CoV-2. It is validated for use with a range of clinical specimens from individuals suspected of respiratory tract infections, including nasopharyngeal swabs (NPS), oropharyngeal swabs (OPS), saliva, sputum, and bronchoalveolar lavage fluid.

Designed as an auxiliary diagnostic tool, this kit supports the diagnosis of influenza and RSV infections when used in conjunction with clinical manifestations and epidemiological risk factors.

For reliable performance, the RPP4D kit is intended exclusively for use by trained clinical laboratory professionals who have received specific instruction and hands-on training in real-time PCR techniques and standard in vitro diagnostic procedures.

Principle

The kit used a multiplex TaqMan probe-based one-step reverse transcription polymerase chain reaction (RT-PCR), which enables simultaneous qualitative detection of influenza A (InfA), influenza B (InfB), respiratory syncytial virus (RSV) and SARS-CoV-2, as well as exogenous internal control (SUC2) in RT-PCR tube A and endogenous internal control (human RNaseP gene) in tube B. For ease of storage and transportation, the amplification reagent is designed in pre-distributed dry form.

CE-IVD Marked