MeltPro® MTB/INH Test Kit

MeltPro® MTB/INH Test Kit

Cat. No.: 801215

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MeltPro® Mycobacterium tuberculosis Isoniazid-resistance Mutation Test Kit

Mycobacterium tuberculosis Isoniazid-resistance Mutation Test is suitable for rapid, qualitative
detection of isoniazid-resistance mutations in Mycobacterium tuberculosis.

Mycobacterium tuberculosis Isoniazid-resistance Mutation Test is based on the Probe-based Melting
Curve Analysis. According to the change in Tm value between targets and the corresponding wild-type
probe sequences, the test detects the mutations in ahpC promoter region (positions -44~-30 and -15~3),
inhA codon 94, inhA promoter region (positions -17~-8), and katG codon 315, which reports
isoniazid-resistance information.

Detection region: mutations in ahpC promoter region (positions -44~-30 and -15~3), inhA codon 94,
inhA promoter region(positions -17~-8) and katG codon 315 (coverage rate: 50%-90% of all isoniazid-resistant mutants)

Principle of Probe-Based Melting Curve Analysis

Probe-based melting curve analysis is based on melting temperature generated by thermal denaturation of
the probe-target hybrid. Melting curve plot of fluorescence (F) versus temperature (T) is transformed into
melting peak by plotting -dF/dT versus temperature. The hybridization probe with fully matched wild-type
target gives the highest Tm value. With matched mutant-type target, it gives the lower T m value.
Mutation is detected as Tm deviation (△Tm ) compared to the wild-type hybrid.

Intended use: CFDA approved in China for clinical use, CE marked for IVD use in Europe.

Features Specifications
Size 48 tests/kit
Sample Cultured Mycobacterium tuberculosis specimens or sputum samples
PCR instrument Zeesan SLAN96
Storage -18°C
Shelf Life 12 months

Please contact for further information.

1. Evaluation of the MeltPro TB STR assay for rapid detection of streptomycin resistance in MTB   Tuberculosis 95 (2015) 162e169, Elsevier Ltd, 2015

2. Drug-Resistant Tuberculosis Test From China’s Zeesan Biotech Performs Well in Clinical Validation    GenomeWeb, 2016

3. Rapid diagnosis of MDR and XDR tuberculosis with the MeltPro TB assay in China     Scientific Reports | 6:25330 | DOI: 10.1038/srep25330, Nature, 2016



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