SARS-CoV-2 Variant P.1 Identification Kit
This kit is only used for in vitro diagnosis by professionals in BSL-2 laboratories. It is used for qualitative detection of SARS-CoV-2 and rapid identification of SARS-CoV-2 variant P.1 in samples, including nasopharyngeal swab, oropharyngeal swab, saliva, bronchoalveolar lavage fluid and sputum.
Principle of the Procedures:
The kit used one-step reverse transcription polymerase chain reaction (One-step RT-PCR) combined with “multicolor probe melting curve analysis” (MMCA®) technique to simultaneously detect three characteristic mutation sites named N501Y, K417T, and E484K that represent the SARS-CoV-2 variant P.1 strain. And the detection results were determined according to the Tm value of the three mutation sites.
The kit can be operated by the real-time PCR thermal cycler with FAM, HEX, and ROX channels. Among them, SLAN 96S,SLAN 96P, SLAN 48P is highly recommended. ABI QuantStudio™ series, MA-6000, LightCycler 480 and Bio-Rad CFX96 need to be tested prior to use.
The kit has obtained CE certification and is applying for Chinese export whitelist. We can export a small amount before getting the Chinese export whitelist.
1) The limit of detection for the kit is: 200 copies/mL.
2) Repeatability: the precision references were tested, the results were all SARS-CoV-2 variant P.1 positive, and the CV of
Tm values is no higher than 5%.