MeltPro® MTB/STR Test Kit

MeltPro® MTB/STR Test Kit

Cat.No.: 801217

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MeltPro® Mycobacterium tuberculosis Streptomycin-resistance Mutation Test Kit

Mycobacterium tuberculosis Streptomycin-resistance Mutation Test is suitable for rapid, qualitative de-
tection of streptomycin-resistance mutations in Mycobacterium tuberculosis.

Mycobacterium tuberculosis Streptomycin-resistance Mutation Test is based on the Probe-based Melting
Curve Analysis. According to the change in Tm value between targets and the corresponding wild-type
probe sequences, the test detects mutations in rpsL codon 43 and codon 88, rrs positions 513~517 and
positions 905~908, which reports streptomycin-resistance information. For each specimen, two tubes are
needed for the test, corresponding to the STR PCR Mix A and Mix B.

Detection region: mutations in rpsL codon 43 and codon 88, rrs positions513~517 and positions 905~908
(mutation rate 70%~90%)

Principle of Probe-Based Melting Curve Analysis

Probe-based melting curve analysis is based on melting temperature generated by thermal denaturation of
the probe-target hybrid. Melting curve plot of fluorescence (F) versus temperature (T) is transformed into
melting peak by plotting -dF/dT versus temperature. The hybridization probe with fully matched wild-type
target gives the highest Tm value. With matched mutant-type target, it gives the lower Tm value.
Mutation is detected as Tm deviation (△Tm ) compared to the wild-type hybrid.

 Intended Use:  CE marked for IVD use in Europe.

Features Specifications
Size 48 tests/kit
Sample Cultured Mycobacterium tuberculosis specimens or sputum samples
PCR instrument Zeesan SLAN96, Bio-Rad CFX96, Rotor-Gene 6000, Roche LC480 II
Storage -18°C
Shelf Life 12 months

Please contact info@zsandx.com for further information.

1. Evaluation of the MeltPro TB STR assay for rapid detection of streptomycin resistance in MTB   Tuberculosis 95 (2015) 162e169, Elsevier Ltd, 2015

2. Drug-Resistant Tuberculosis Test From China’s Zeesan Biotech Performs Well in Clinical Validation    GenomeWeb, 2016

3. Rapid diagnosis of MDR and XDR tuberculosis with the MeltPro TB assay in China     Scientific Reports | 6:25330 | DOI: 10.1038/srep25330, Nature, 2016

 

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