MeltPro® MTB/SL Test Kit
MeltPro® Mycobacterium tuberculosis Second-line Injectable Drug-resistance Mutation Test Kit
Mycobacterium tuberculosis Second-line Injectable Drug-resistance Mutation Test is suitable for rapid,
qualitative detection of second-line injectable drug-resistance mutations in Mycobacterium tuberculosis.
Mycobacterium tuberculosis Second-line Injectable Drug-resistance Mutation Test is based on the
Probe-based Melting Curve Analysis. According to the change in Tm value between targets and the
corresponding wild-type probe sequences, the test detects the mutations in rrs (positions 1401, 1402 and 1484) and eis promoter region (positions -37, -14 and -10), which reports second-line
injectable drug-resistance information.
Principle of Probe-Based Melting Curve Analysis
Probe-based melting curve analysis is based on melting temperature generated by thermal denaturation of
the probe-target hybrid. Melting curve plot of fluorescence (F) versus temperature (T) is transformed into
melting peak by plotting -dF/dT versus temperature. The hybridization probe with fully matched wild-type
target gives the highest Tm value. With matched mutant-type target, it gives the lower Tm value.
Mutation is detected as Tm deviation (△Tm ) compared to the wild-type hybrid.
Intended Use: CE marked for IVD use in Europe.
|Sample||Cultured Mycobacterium tuberculosis specimens or sputum samples|
|PCR instrument||Zeesan SLAN96, Bio-Rad CFX96, Rotor-Gene 6000, Roche LC480 II|
|Shelf Life||12 months|
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1. Evaluation of the MeltPro TB STR assay for rapid detection of streptomycin resistance in MTB Tuberculosis 95 (2015) 162e169, Elsevier Ltd, 2015
2. Drug-Resistant Tuberculosis Test From China’s Zeesan Biotech Performs Well in Clinical Validation GenomeWeb, 2016
3. Rapid diagnosis of MDR and XDR tuberculosis with the MeltPro TB assay in China Scientific Reports | 6:25330 | DOI: 10.1038/srep25330, Nature, 2016