Lab-Aid Virus RNA Extraction Kit
Lab-Aid Virus RNA Extraction Kit utilizes magnetic beads technology for automated isolation and purification of genomic
RNA from nasopharyngeal swab, oropharyngeal swab, sputum, bronchoalveolar lavage fluid (BALF) and stool swab. The
product is intended to be used by professional users, such as technicians and physicians who are trained in molecular biological
techniques. The kit provides high-quality RNA that is suitable for direct use in downstream applications, such as amplification
or other enzymatic reactions. The Lab-Aid series isolation system performs all steps of the sample preparation procedure for
up to 24 samples in a single run.
Priciple of The Procedures:
Magnetic-beads technology combines the speed and efficiency of silica-based nucleic acid purification with the convenient handling of magnetic beads. The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples. The purification procedure comprises 4 steps: lysis, binding, washing, and elution.
Lysis: The samples are lysed first in the presence of lysis buffer, which allows the RNA to be released.
Binding: Binding buffer is added to the lysed samples to adjust binding conditions. Lysates are thoroughly mixed with
magnetic beads to allow optimal adsorption of RNA to the silica surface. Salt and pH conditions ensure that protein and other
contaminants, which can inhibit PCR and other downstream enzymatic reactions, are not bound to the magnetic beads.
Washing: While the RNA remains bound to the magnetic beads, contaminants are efficiently washed away during a sequence
of wash steps using wash buffer R1, then wash buffer R2.
Elution: Highly pure RNA is eluted in elution buffer. The purified RNA can be either used immediately in downstream
applications or stored for future use.