SARS-CoV-2 Variant B.1.1.7 Identification Kit

Intended Use:

This kit is only used for in vitro diagnosis by professionals in BSL-2 laboratories. It is used for qualitative detection of SARS-CoV-2 and rapid identification of SARS-CoV-2 variant B.1.1.7 in samples, including nasopharyngeal swab, oropharyngeal swab, saliva, bronchoalveolar lavage fluid and sputum.

Principle of the Procedures:

The kit used one-step reverse transcription polymerase chain reaction (One-step RT-PCR) combined with “multicolor probe melting curve analysis” (MMCA®) technique to simultaneously detect four characteristic mutation sites named N501Y, D614G, HV69-70 del and Y144 del that represent the SARS-CoV-2 variant B.1.1.7 strain. And the detection results were determined according to the Tm value of the four mutation sites.

Instument:

The kit can be operated by the real-time PCR thermal cycler with FAM, HEX, ROX and CY5 channels. Among them, SLAN 96S, SLAN 96P, SLAN 48P is highly recommended. ABI QuantStudio™ series, MA-6000, LightCycler 480 and Bio-Rad CFX96 need to be tested prior to use.

The kit has obtained CE certification and Chinese export whitelist, and can be exported in large quantities.